Improvement of poly(3-hydroxybutyrate) [P(3HB)] production in Corynebacterium glutamicum by codon optimization, point mutation and gene dosage of P(3HB) biosynthetic genes.

The highest production of P(3HB) was achieved by combining the gene dosage of phaAB Re with codon optimization and the replacement of wild-type phaC Re with a modified gene encoding a mutation of Gly4Asp (G4D), which enhanced the production of PhaC re and P( 3HB) in Escherichia coli.